条形柄锈菌34号生理小种的分子标记

条形柄锈菌Puccinia striiformis f. sp. tritici 34号生理小种(CYR34)是目前我国毒性谱最宽、毒性最强的生理小种,对小麦生产和抗病品种选育造成了极大的影响。本研究采用RAPD-SCAR分子标记技术,从300条RAPD随机引物中筛选到CYR34的特异引物,通过特异性片段回收、克隆和测序(GenBank登录号为OL907303),依据序列设计出了S2008F34/S2008R34特异性引物,能够从CYR34及接种CYR34的小麦发病叶片总DNA中都扩增出417 bp的目标片段。采用该特异性引物检测2021年陕西渭南、咸阳和宝鸡地区小麦条锈菌CYR34的流行频率分别为8.6%、6.0%和10.8%。该项研究为小麦条锈菌CYR34号生理小种的快速检测提供了技术支撑。

Molecular marking of Puccinia striiformis f. sp. tritici race CYR34

Chinese Yellow Rust 34 (CYR34) is currently the physiological race with the broadest virulent spectrum and the highest virulence of Puccinia striiformis f. sp. tritici, which is a serious threat to the production and breeding of wheat in China. One specific primer among 300 random RAPD primers was screened to differentiate the CYR34 of P. striiformis f. sp. tritici from other races. The specific band was purified from the agarose, cloned into pMD18-T vector, and sequenced (GenBank accession: OL907303). Specific primer pair of S2008F34/S2008R34 was designed based on the sequence, which could amplify a 417 bp specific band from both the genome DNA of CYR34 and the total DNA of wheat leaves inoculated with CYR34. The specific primer pair was used to detect the epidemic frequency of CYR34 in Weinan, Xianyang, and Baoji, Shaanxi Province in 2021. The results showed that the epidemic frequency of CYR34 were 8.6%, 6.0%, and 10.8%, respectively. The present study provides a technical assistance for rapid detection of CYR34 of P. striiformis f. sp. tritici.