| 固相萃取-超高效液相色谱-串联质谱法测定新鲜块菌子实体中α-雄烷醇 |
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| 引用本文： | 程池露,李传华,熊海宽,冯娜,张劲松.固相萃取-超高效液相色谱-串联质谱法测定新鲜块菌子实体中α-雄烷醇[J].菌物学报,2022,41(5):830-836. |
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| 作者姓名： | 程池露 李传华 熊海宽 冯娜 张劲松 |
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| 作者单位： | 1 上海海洋大学食品学院,上海 2013062 上海市农业科学院食用菌研究所 农业部南方食用菌资源利用重点实验室 国家食用菌工程技术研究中心 国家食用菌加工技术研发分中心 上海市农业遗传育种重点开放实验室,上海 2014033 永仁野森达菌业有限公司,云南 楚雄 651499 |
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| 基金项目： | 云南省张劲松专家工作站(20210SAF150009) |
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| 摘 要： | 本研究建立了一种固相萃取-超高效液相色谱-串联质谱的检测方法,用于检测新鲜块菌子实体中α-雄烷醇(5α-雄甾-16-烯-3α-醇)的含量。新鲜块菌样品经无水乙醇提取,Qasis HLB柱萃取富集后,采用超高效液相色谱-串联质谱进行分析定量。方法学验证结果表明该方法的回收率为88.49%-92.22%;检出限为0.120 9 ng/mL,定量限为0.398 9 ng/mL。该方法简便、精确,适用于新鲜块菌中α-雄烷醇含量的测定。
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| 关 键 词： | 块菌 α-雄烷醇 超高效液相色谱-串联质谱 |
| 收稿时间： | 2021-11-18 |
Determination of α-androstanol in fresh Tuber indicum fruiting bodies by solid phase extraction-ultra performance liquid chromatography-tandem mass spectrometry |
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| Authors: | CHENG Chilu LI Chuanhua XIONG Haikuan FENG Na ZHANG Jingsong |
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| Abstract: | A innovative method using solid-phase extraction combined with ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS2) was developed to determine α-androstanol (5α-androst-16-en-3α-ol) in fresh fruiting bodies of Tuber indicum. The α-androstanol in fruiting bodies were extracted with ethanol, purified by solid phase cartridges, and then analyzed and quantified by UPLC-MS2. Method logical validation results showed that the recovery rates of α-androstanol by using the method are 88.49%-92.22%; the detection limit is 0.120 9 ng/mL, and the quantification limit is 0.398 9 ng/mL. This method was rapid and accurate, and suitable for the determination of α-androstanol content in fresh fruiting bodies of Tuber indicum. |
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| Keywords: | Tuber indicum α-androstanol ultra-high performance liquid chromatography-tandem mass spectrometry |
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