促白木香结香真菌的快速筛选

以海南省澄迈县海南科大林业公司所有的白木香人工林地为研究对象,采用前期已分离的菌株对白木香白木进行固体催化,利用GC-MS技术对具有催化效果(白木化学成分在催化前后变化显著)的菌株进行筛选,将筛选出的菌株接种至白木香树体中,6个月后对沉香物质挥发油以及化学成分进行测定。对白木固体催化产物的提取物分析,鉴定出其乙醚提取物共24种化学成分,其中共有成分5种、芳香族类化合物6种、其他类化合物18种。Trichoderma sp.、Neurospora sp.和Melanotus flavolivens这3种菌株使白木香白木的化学成分发生显著变化。对结香处理6个月后已结香木材的提取物分析,鉴定出其乙醚提取物共84种化学成分,其中共有成分30种,芳香族类化合物17种、倍半萜类化合物34种和其他类化合物33种。Trichoderma sp.、Neurospora sp.、Melanotus flavolivens和CK样品乙醚提取物得率分别为3.62%、4.04%、3.97%和1.94%。其中芳香族类成分相对含量之和分别为15.15%、17.29%、12.13%和7.95%。倍半萜类成分相对含量之和分别为10.61%、11.19%、11.4%和0%。在6个月内Trichoderma sp.、Neurospora sp.和Melanotus flavolivens这3种经过白木香白木固体催化筛选所得的菌株能够有效诱导白木香结香。

Rapid screening of fungi that promote the production of agarwood in Aquilaria sinensis

Taking the Aquilaria sinensis plantation owned by the HAINAN KEDA Forestry Co., Ltd. in Chengmai County, Hainan Province as experimental woodland, the solid catalysis of (Aquilaria sinensis) was carried out by using the fungal strains isolated previously, and GC-MS technology was used to screen out the strains having catalytic effect. It is found that the chemical composition of agarwood changes significantly after catalysis. The screened strains with catalytic effect were inoculated to the Aquilaria sinensis, and the volatile oil and chemical components of agarwood were determined in 6 months after inoculation. The analysis of the extracts of the solid catalytic products of resinated wood showed that there were 24 chemical constituents in the ether extract, including 5 constituents in common, 6 aromatic compounds and 18 other compounds. The three strains, Trichoderma sp., Neurospora sp. and Melanotus flavolivens, significantly changed the chemical composition of agarwood. The analysis of the extracts of the incense wood in 6 months after treatment showed that there were a total of 84 chemical components in the ether extract, including 30 components in common, 17 aromatic compounds, 34 sesquiterpenoids compounds and 33 other compounds. The extraction rate of ether extract of the samples treated with Trichoderma sp., Neurospora sp., Melanotus flavolivens and CK samples were 3.62%, 4.04%, 3.97% and 1.94%, respectively. The sums of relative content of aromatic components were 15.15%, 17.29%, 12.13% and 7.95%, respectively. The sums of relative content of sesquiterpenoids were 10.61%, 11.19%, 11.4% and 0%, respectively. Trichoderma sp., Neurospora sp. and Melanotus flavolivens screened through solid catalysis of resinated wood can effectively induce Aquilaria sinensis to generate agarwood within 6 months.