红托竹荪多糖对大鼠酒精性肝损伤的保护作用

研究红托竹荪多糖(Dictyophora rubrovalvata polysaccharide,DRP)对酒精所致大鼠肝损伤的保护作用。采用苯酚-硫酸法测得DRP的含量为74.68%±1.32%，利用傅里叶红外光谱初步分析表明DRP是含有α-糖苷键和β-糖苷键的吡喃环多糖。当DRP浓度达到3.0 mg/m L时，DPPH自由基的清除率达到80.12%，其还原力为0.31，对羟基自由基的清除率达到88.07%。雄性SD大鼠被随机分为6组：空白对照组(NC)、模型对照组(MC)、阳性对照组(PC)、红托竹荪多糖低(LDRP)、中(MDRP)、高(HDRP)剂量干预组，连续灌胃28 d后将其安乐死，测定血清中AST、ALT、TG水平以及肝脏SOD、GSH、MDA、TNF-α、IL-6水平，并根据病理切片分析红托竹荪多糖对大鼠酒精性肝损伤的保护程度。与MC组相比，DRP各剂量组血清AST、ALT、TG水平显著降低(P<0.05)，肝脏SOD和GSH水平显著上升(P<0.05),MDA、TNF-α、IL-6含量显著下降(P<0.05)，肝脏细胞变性和坏死等病理现象明显改善。D...

Protective effects of Dictyophora rubrovalvata polysaccharide on alcoholic liver injury in rats

The protective effects of Dictyophora rubrovalvata polysaccharides (DRP) on rats with alcoholic liver injury were investigated. The content of DRP determined by phenol sulfuric acid method was 74.68%±1.32%. The preliminary analysis using Fourier infrared spectroscopy showed that DRP contained α-glycosidic and β-glycosidic linked pyranocyclic polysaccharides. When the concentration of DRP reached 3.0 mg/mL, the scavenging rate of DPPH radical reached 80.12%, the reducing power reached 0.31, and the scavenging rate of hydroxyl radical reached 88.07%. Male SD rats were randomly divided into six groups: normal control (NC), model control (MC), positive control (PC), low-dose (LDRP), middle-dose (MDRP), and high-dose D. rubrovalvata polysaccharides (HDRP) groups. After continuous intragastric administration for 28 d, all rats were euthanized. The serum AST, ALT and TG were examined, and the levels of SOD, GSH, MDA, TNF-α, IL-6 in livers were assayed. The protective effects of D. rubrovalvata polysaccharide (DRP) on alcoholic liver injury in rats was analyzed according to pathological sections. Compared with the MC group, the levels of AST, ALT and TG in the DRP intervention groups were significantly decreased (P<0.05), the levels of SOD and GSH in liver were significantly increased (P<0.05), the contents of MDA, TNF-α and IL-6 in liver were significantly decreased (P<0.05). The pathological phenomena of liver cellular degeneration and necrosis were improved obviously. DRP has a certain antioxidant capacity in vitro, and can alleviate alcohol-induced liver injury in rats, and its alleviating effect is related to the enhancement of antioxidant capacity and the decrease of the expression level of inflammatory factors.