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Phytohormones Accumulation and Distribution in Shoots and Roots of Haploid,Diploid and Tetraploid Barley Seedlings Derived from Microspore Culture
Authors:Longhua Zhou  Ting He  Jing Li  Guimei Guo  Yingbo Li  Hongwei Xu  Runhong Gao  Linli Huang  Yifei Wang  Ruiju Lu  Zhiwei Chen  Chenghong Liu
Institution:1 Biotech Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, 200000, China2 Biotechnology Research Institute, Shanghai Key Laboratory of Agricultural Genetics and Breeding, Shanghai, 200000, China3 Shanghai Baixin Biotechnology Company Limited, Shanghai, 200000, China
Abstract:Phytohormones play important roles in plant growth and development, and polyploids are thought to be an important method for plant breeding. However, the relationship between ploidy and phytohormone is still unclear. In this study, barley at three ploidy levels were produced by microspore culture. Therefore, we further analyzed the phytohormone content in the shoots and roots of the three kinds of barley materials to study the effect of ploidy on phytohormones accumulation and distribution. The results showed that Abscisic acid (ABA), gibberellin (GA), jasmonic acid (JA), auxin (IAA), salicylic acid (SA) and cytokinin (CTK) were successfully determined in shoots and roots using LC-MS (liquid chromatography mass spectrometry). By comparing the shoots of the haploid and diploid plants, it was found that the distribution trend of the six phytohormones was consistent, and another consistent trend was found in the roots of the diploid and tetraploid plants. In addition, we further analyzed the shoot/root ratio of the different phytohormones to identify the potential differences for haploid, diploid and tetraploid. Here, the relationship between ploidy and phytohormone we provided would provide new insights into understanding the new phenotypes that occur in polyploid species.
Keywords:Barley  polyploidy  microspore culture  phytohormone  shoot  root
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