黑木耳子实体粉代替部分膳食对高脂模型小鼠营养性肥胖预防作用

本文旨在研究黑木耳粉代替部分膳食对模型小鼠预防营养性肥胖的作用功效。试验选择5周龄健康ICR雄性小鼠48只,随机分为正常对照组(CK)、高脂组(HFD)、阳性药物对照组(PD)和黑木耳粉添加组(AH)。连续饲喂9周,检测小鼠的各项生理生化指标。结果表明：与HFD组相比,AH组小鼠体重、附睾脂肪指数显著降低(P<0.05);Lee’s指数、血清葡萄糖(GLU)含量、甘油三酯(TG)含量和肝脏指数含量极显著降低(P<0.01),血清高密度脂蛋白胆固醇(HDL-C)和肝脏HDL-C含量极显著提高(P<0.01);染色结果观察到AH组小鼠肝脏中脂肪细胞减小,脂质含量降低;附睾脂肪体积减小,附睾细胞形状较规则。高通量测序分析发现,黑木耳粉干预有效改善了小鼠肠道中的变形菌门、螺杆菌属与拟杆菌属的相对丰度。研究结果表明黑木耳有显著预防小鼠营养性肥胖的功效,为黑木耳的开发利用提供依据。

Preventive effects of powder of Auricularia heimuer fruiting bodies on nutritional obesity of high-fat model mice

The purpose of this study was to explore the effect of Auricularia heimuer fruiting body powder (AH) replacing diet in part on preventing nutritional obesity of high-fat model mice. Forty eight five-week-old ICR male mice were randomly divided into normal control group (CK), high fat treatment group (HFD), positive drug control group (PD) and AH treatment group. After nine weeks of feeding, the physiological and biochemical indicators of mice were measured. Compared with HFD group, results showed that the body weight and epididymis fat index of AH group were significantly decreased (P<0.05), and the Lee’s index, the serum glucos (GLU), triglyceride (TG) content and the liver index were significantly decreased (P<0.01). Meanwhile, the serum high-density lipoprotein cholesterol (HDL-C) and the liver HDL-C contents were significantly increased (P<0.01). HE staining indicated that the lipid content and the adipocyte size in liver were decreased, the fat volume of epididymis was also decreased, and the shape of epididymis cells was regular in the AH group. High-flux sequencing analysis showed that the relative abundance of Proteobacteria, Helicobacter and Bacteroides in the intestinal tract of mice were improved after the intervention of AH powder. The results indicated that AH had a significant effect on preventing nutritional obesity of mice, and providing a basis for the development and utilization of AH.