海洋真菌Penicillium sp. WP-13次级代谢产物研究

本研究分析了海洋真菌Penicillium sp. WP-13的活性次级代谢产物。采用多种柱色谱技术对其发酵液的乙酸乙酯提取物进行分离纯化；根据波谱数据和理化常数分析，并结合文献比对鉴定单体化合物的结构；采用MTT法测定化合物对肿瘤细胞的细胞毒活性。从Penicillium sp. WP-13发酵液的乙酸乙酯提取物中分离鉴定了5个单体化合物，其中2个内酯类化合物为1-hydroxy-3,10-dimethoxy-8-methyl-6,11-dioxo-6,11-dihydrodibenzob,e]oxepine-9-carboxylicacid(1)和1,8-dihydroxy-10-methoxy-3-methyl-dibenzob,e]oxepine-6,11-dione(2); 3个蒽醌类化合物为endocrocinmethylester(3)、2-chloro-1,3,8-trihydroxy-6-methyl-9,10-anthracenedione (4)和emodin (5)。活性测试结果显示，化合物3和5均对人慢性髓原白血病细胞株K562、人肝癌细胞株BEL-740...

Secondary metabolites of marine-derived fungus Penicillium sp. WP-13

The active secondary metabolites of the marine-derived fungus Penicillium sp. WP-13 were investigated. The secondary metabolites were isolated and purified by various column chromatography techniques, and the structures of the compounds were identified by analysis of spectral data and physicochemical constants combined with literature comparison. The cytotoxic activity of the compounds was evaluated by MTT assay. Five compounds including two lactones and three anthraquinones were isolated from the ethyl acetate extract of the fermentation broth of Penicillium sp. WP-13. Their structures were determined as 1-hydroxy-3,10-dimethoxy-8- methyl-6,11-dioxo-6,11-dihydrodibenzob,e]oxepine-9-carboxylic acid (1), 1,8-dihydroxy-10- methoxy-3-methyl-dibenzob,e]oxepine-6,11-dione (2), endocrocin methyl ester (3), 2-chloro- 1,3,8-trihydroxy-6-methyl-9,10-anthracenedione (4), and emodin (5). The results of cytotoxic test showed that compounds 3 and 5 displayed cytotoxic activity against human chronic myeloid leukemia cell line K562, human liver cancer cell line BEL-7402, human gastric cancer cell line SGC-7901 and human cervical cancer cell line HeLa. Compound 1 is a new lactone, and the NMR data of compound 3 were reported for the first time. Compounds 1-4 were isolated from Penicillium for the first time.