西藏昆诺阿藜种子可培养内生真菌多样性

探究西藏不同地区昆诺阿藜种子内生真菌的物种组成、多样性等群落结构特征,丰富昆诺阿藜种子相关微生物的可利用资源,对于微生物资源利用和昆诺阿藜病害的生物防控具有重要意义。本研究收集西藏不同海拔3个地区(日喀则、拉萨和林芝)的昆诺阿藜种子,利用传统分离方式进行了真菌的培养。从46份昆诺阿藜种子中共分离出947株真菌,经ITS序列分析结合形态观察鉴定为1门9目12科26属77种。所有的真菌都归类为子囊菌门Ascomycota,菌株数量相对丰度最高的3个属为链格孢属Alternaria (40.2%)、镰刀菌属Fusarium (17.4%)和茎点霉属Phoma (13.9%)。按照多样性指数,日喀则地区的昆诺阿藜内生可培养真菌的多样性明显高于拉萨和林芝两个地区。

Culturable endophytic fungal diversity of quinoa seeds in Tibet,Southwest China

The diversity and community structure of endophytic fungi in quinoa seeds from different areas in Tibet were explored for the purpose of providing the basis for utilization of microbial resources in biocontrol practice. The cultivable fungi in seeds of quinoa collected from three areas of Tibet with different altitudes were isolated using traditional methods. In total 947 fungal strains were isolated from 46 quinoa seed samples and they were identified as 77 species belonging to 26 genera, 12 families, 9 orders, 1 phylum by ITS sequence analysis and morphological observation. All the fungi belonged to Ascomycota, and the three most dominant genera were Alternaria (isolation frequency 40.2%), Fusarium (17.4%), and Phoma (13.9%). Diversity of endophytic culturable fungi in quinoa seeds in Shigatse region was significantly higher than that in Lhasa and Nyingchi regions.