| 正交试验优化花魔芋组织培养条件 |
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| 引用本文： | 陈永波,赵清华,滕建勋,钟刚琼. 正交试验优化花魔芋组织培养条件[J]. 氨基酸和生物资源, 2005, 27(2): 29-31. DOI: 10.3969/j.issn.1006-8376.2005.02.010 |
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| 作者姓名： | 陈永波 赵清华 滕建勋 钟刚琼 |
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| 作者单位： | 恩施州农科院魔芋研究所,恩施,445000;恩施州农科院魔芋研究所,恩施,445000;恩施州农科院魔芋研究所,恩施,445000;恩施州农科院魔芋研究所,恩施,445000 |
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| 基金项目： | 湖北省西部少数民族科技开发项目;2004AA210B02; |
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| 摘 要： | 通过正交试验优化了魔芋愈伤组织生长及出芽的条件:以花魔芋切块为外植体,在MS(或1/2MS)+6-BA1.5+NAA0.5+IBA1.0+糖30%(pH6.2)的培养基上,暗培养15～20d后,光照12h/d,不仅愈伤组织生长旺盛,而且可以一步成苗,形成完整的植株,能够按要求分批生产试管苗。
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| 关 键 词： | 魔芋 组织培养 正交试验 愈伤组织 试管苗 |
| 文章编号： | 1006-8376(2005)02-0029-02 |
| 修稿时间： | 2004-11-16 |
Optimized Tissue Culture Conditions of Amorphophallus konjac K Koch by Orthogonal Experimentation |
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| CHEN Yong-bo,ZHAO Qing-hua,TENG Jiang-xun,ZHONG Gang-qiong. Optimized Tissue Culture Conditions of Amorphophallus konjac K Koch by Orthogonal Experimentation[J]. Amino Acids & Biotic Resources, 2005, 27(2): 29-31. DOI: 10.3969/j.issn.1006-8376.2005.02.010 |
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| Authors: | CHEN Yong-bo ZHAO Qing-hua TENG Jiang-xun ZHONG Gang-qiong |
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| Abstract: | The tissue culture conditions of Amorphophallus konjac K Koch were optimized by orthogonal experimentation. The tuber slices were used as explants for tissue culture. The explants were inoculated in MS (or 1/2MS) media +1.5mg/L 6-BA+0.5 mg/L NAA+1.0 mg/L IBA+30% sugar(pH6.2). After 15-20 days in the dark, the sample was illuminated for 12 hours per day. The callus grew rapidly and could differentiate buds in the same media. The tube seedling could be produced. |
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| Keywords: | Amorphophallus konjac K Koch tissue culture orthogonal experimentation callus tube seedling |
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