Expression of human serum albumin in methylotrophic yeast Pichia pastoris and its structural and functional analysis |
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| Authors: | T V Bobik I I Vorob’ev N A Ponomarenko A G Gabibov A I Miroshnikov |
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| Institution: | (1) Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117997, Russia |
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| Abstract: | The stable strain of methylotrophic yeast Pichia pastoris secreting human serum albumin into cultural medium was obtained. Optimal conditions for expression of the protein were determined. We characterized the recombinant protein by mass spectrometry and circular dichroism and analyzed its catalytic activity. |
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| Keywords: | expression human serum albumin Pichia pastoris |
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