|
|||||
|
|
New application of Aspergillus versicolor in promoting plant growth after suppressing sterigmatocystin production via genome mining and engineering |
|
| Authors: | Siqi Ma Donglin Zhao Xiaobin Han Yulong Peng Tingting Ren Mei Wang Jun Wan Jilin Ding Xiuchun Du Fubin Zhao Yiqiang Li Chengsheng Zhang |
| Affiliation: | 1. Marine Agriculture Research Center, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, China;2. Marine Agriculture Research Center, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, China Contribution: Conceptualization (equal), Data curation (equal), Formal analysis (equal), Funding acquisition (equal), Investigation (equal), Methodology (equal), Project administration (equal), Resources (equal), Software (equal), Supervision (equal), Validation (equal), Visualization (equal), Writing - original draft (equal), Writing - review & editing (equal);3. Zunyi Branch, Guizhou Tobacco Company, Zunyi, China Contribution: Conceptualization (equal), Data curation (equal), Formal analysis (equal), Funding acquisition (equal), Investigation (equal), Methodology (equal), Project administration (equal), Supervision (equal), Writing - original draft (supporting), Writing - review & editing (equal);4. Zunyi Branch, Guizhou Tobacco Company, Zunyi, China Contribution: Data curation (equal);5. Marine Agriculture Research Center, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, China Contribution: Data curation (equal);6. Jiaozhou Branch of Qingdao Tobacco Co. Ltd., Qingdao, China Contribution: Data curation (equal);7. Huangdao Branch of Qingdao Tobacco Co. Ltd., Qingdao, China Contribution: Data curation (equal) |
| Abstract: | Aspergillus genus is a key component in fermentation and food processing. However, sterigmatocystin (STE)—a mycotoxin produced by several species of Aspergillus—limits the use of some Aspergillus species (such as Aspergillus versicolor, Aspergillus inflatus, and Aspergillus parasiticus) because of its toxicity and carcinogenicity. Here, we engineered an STE-free Aspergillus versicolor strain based on genome mining techniques. We sequenced and assembled the Aspergillus versicolor D5 genome (34.52 Mb), in which we identified 16 scaffolds and 54 biosynthetic gene clusters (BGCs). We silenced cytochrome P450 coding genes STC17 and STC27 by insertional inactivation. The production of STE in the Δstc17 mutant strain was increased by 282% but no STE was detected in the Δstc27 mutant. Metabolites of Δstc27 mutant exhibited growth-promoting effect on plants. Our study makes significant progress in improving the application of some Aspergillus strains by restricting their production of toxic and carcinogenic compounds. |
| Keywords: | |