Differential gene expression during somatic embryogenesis in Coffea arabica L., revealed by RT-PCR differential display |
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| Authors: | R Rojas-Herrera F Quiroz-Figueroa M Monforte-González L Sánchez-Teyer V M Loyola-Vargas |
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| Institution: | (1) Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, Apartado Postal 87, Cordemex, Mérida, Yucatán, México;(2) Departamento de Genética y Mejoramiento, Instituto Nacional de Ciencias Agrícolas. Gaveta Postal 1. San José de las Lajas, La Habana, Cuba |
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| Abstract: | Molecular and biochemical studies of somatic embryogenesis may help to shed light on the mechanisms governing this phenomenon.
In this article, a differential display analysis approach was employed to investigate the changes taking place during the
induction of somatic embryogenesis in leaf explants and suspension cultures of coffee. Cloned fragments show homologies to
several proteins reported in databases, but only one has previously been described as regulated during somatic embryogenesis.
By a reverse dot blot modification, the expression pattern of such fragments was evaluated. |
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| Keywords: | Coffea arabica somatic embryogenesis gene expression differential display |
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