Sequence and characterization of twoHSP70 genes in the colonial protochordateBotryllus schlosseri |
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| Authors: | Melinda B Fagan Irving L Weissman |
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| Institution: | (1) Department of Pathology, Stanford University Medical Center, Stanford University, 94305 Stanford, CA, USA;(2) Present address: Department of Biological Sciences, Stanford University, 94305 Stanford, CA, USA;(3) Present address: Department of Pathology, Stanford University School of Medicine, Stanford University Medical Center, 94305-5524 Stanford, CA, USA |
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| Abstract: | Two genes belonging to the heat shock protein 70 gene family have been cloned from the colonial protochordateBotryllus schlosseri. The two intronless genes(HSP70.1 andHSP70.2) exhibit 93.6% sequence identity within the predicted coding region, and 83.3% and 81.7% sequence identity in the 5′ and 3′
flanking regions, respectively. The predicted amino acid sequences are 95% identical and contain several signatures characteristic
of cytoplasmic eukaryoticHSP70 genes (Gupta et al. 1994; Rensing and Maier 1994). Northern blotting and sequence analysis suggest that both genes are heat-inducible
merebees of theHSP70 gene family. Given these characteristics,HSP70.1 andHSP70.2 appear to be good candidates for protochordate homologues of the major histocompatibility complex-linkedHSP70 genes of human, mouse, and rat (Milner and Campbell 1990; Walter et al. 1994). Further experiments to determine whether there
is functional evidence for such similarity are in progress.
The nucleotide sequence data reported in this paper have been submitted to the EMBL/GenBank nucleotide sequence databases
and have been assigned the accession numbers US 1901 (HSP70.1) and US 1902 (HSP70.2) |
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