| RP-HPLC测定红丝线提取物中紫蓝素的含量 |
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| 引用本文： | 蒋小华,谢运昌,黄永林.RP-HPLC测定红丝线提取物中紫蓝素的含量[J].广西植物,2007,27(6):958-960. |
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| 作者姓名： | 蒋小华 谢运昌 黄永林 |
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| 作者单位： | 广西壮族自治区,中国科学院,广西植物研究所,广西,桂林,541006 |
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| 基金项目： | 国家自然科学基金
,
广西自然科学基金
,
国家科技攻关计划西部开发重大项目
,
广西科技基金 |
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| 摘 要： | 建立了红丝线提取物中紫蓝素的测定方法。采用反相高效液相色谱法,色谱柱为ZORBAXXDB-C18(4.6mm×150mm,5μm);流动相:V(乙腈):V75mmol/L乙酸铵+0.5mmol/LEGTA(pH7.0)]=8∶92;流速:1mL/min;检测波长590nm。紫蓝素的线性范围为2.5～50mg/L(r=0.9999),回收率97.9%～101.5%。该法简便、准确,重复性好,适用于测定红丝线提取物中紫蓝素的含量。
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| 关 键 词： | 高效液相色谱法 紫蓝素 红丝线提取物 |
| 文章编号： | 1000-3142（2007）06-0958-03 |
| 收稿时间： | 2006-05-12 |
| 修稿时间： | 2007-01-25 |
Analysis of zilanine in extract of Peristrophe
baphica by RP-HPLC |
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| JIANG Xiao-Hu,XIE Yun-Chang,HUANG Yong-Lin.Analysis of zilanine in extract of Peristrophe
baphica by RP-HPLC[J].Guihaia,2007,27(6):958-960. |
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| Authors: | JIANG Xiao-Hu XIE Yun-Chang HUANG Yong-Lin |
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| Institution: | Guangxi Institute of Botany, Guangxi Zhuangzu Autonomous Region and the Chinese Academy of Sciences, Guilin 541006, China |
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| Abstract: | The paper established a RP-HPLC method for the determination of the content of zilanine in extract of Peristrophe baphica.RP-HPLC was conducted on a ZORBAX XDB-C18(4.6 mm×150 mm,5 μm)with CH3CN-CH3COONH4(75 mmol/L)+EGTA(0.5 mmol/L,pH=7.0)]=8∶92;flow rate 1.0 mL/min,the detection wavelength was set at 590 nm.The linear range of zilanine was 2.5～50 mg/L(r=0.9999).The recoveries were 97.9%～101.5%.The method is simple and accurate with good reproducibility,it is suitable for the determination of zilanine content in extract of P.baphica. |
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| Keywords: | HPLC zilanine extract of Peristrophebaphica |
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