Susceptibility of <Emphasis Type="Italic">Staphylococcus aureus</Emphasis> strains toward combinations of oxacillin-2,4-dihydroxychalcone |
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Authors: | J M Talia M A Alvarez N B Debattista N B Pappano |
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Institution: | (1) Division of Infectious Diseases, Duke University Medical Center, Durham, North Carolina, USA |
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Abstract: | In order to determine the existence of synergism of the bacteriostatic action of flavonoids against G+ bacteria between a clinically interesting conventional antibiotic and a flavonoid, combinations of oxacillin (OXC) and 2,4-dihydroxychalcone
(DCH) as enhancer were assayed against methicillin-sensitive Staphylococcus aureus ATCC 29 213 and methicillin-resistant S. aureus ATCC 43 300. Using a kinetic-turbidimetric method, growth kinetics was monitored in a broth containing variable amounts of
OXC alone and combinations of variable OXC-constant DCH. The minimum inhibitory concentrations (MIC) of OXC alone and in combination
with DCH were evaluated. For the 29 213 strain, OXC MIC was 25 μg/mL, while combinations of 2–8 μg/mL OXC with 10 μg/mL of
DCH totally inhibited growth and showed synergism. The resistance of the 43 300 strain in the presence of OXC was verified;
OXC-DCH combinations decreased bacterial growth by 35 %. DCH augments the action of OXC against methicillin-susceptible S. aureus and therefore constitutes a good bacteriostatic agent for methicillin-resistant S. aureus. |
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