不同类型水稻花药培养力差异的生理背景研究

在相同的培养条件下,比较了不同类型水稻花药培养力的差异,观察到糯大于粳,粳大于籼的梯度。从种子及花药淀粉总量和支链淀粉相对量的测定数据中看到:不同类型水稻花药培养力的差异与它们的支链淀粉含量差异有正相关趋势。从供试材料的支、直链淀粉空间构型的比较分析,又看出支链淀粉在水解和提供呼吸底物方面占有很大优势。这说明支链淀粉的优势,可能是通过培养初期较好的能量代谢与花粉细胞的启动和脱分化后,由配子体途径转入孢子体途径的大量细胞增殖有关。

STUDIES ON PHYSIOLOGICAL BACKGROUND ABOUT DIFFERENCES AMONG ANTHER CULTURE ABILITIES OF RICE

In process of anther culture of rice (Oryza sativa L.), the relationships between differences of anther culture abilities in different rice plant cultivars and the amylopectin content of its anther were found to be that initial energy metabolism plays a decisive role in the initiation and development of pollen grains. According to this, the author approached a new path of improving differentiation frequency of anther culture in rice. A series of experiments were carried out under the same steady condition, comparing the differences between anther culture abilities of different rice types. The results range as: Nuo rice>Keng rice>Hsien rice. The parallel relationship exists between difference of anther culture abilities and amylopectin contents measured in anther of rice. Amylopectin as compared with amylose has some advantages in amylolysis of analyzing the spacial configuration of both the amylose and the amylopectin. By measuring the respiratory iptensity of anther in culture, there was no difference in amylopectin of anther. It is proved that the initiation and development of the pollen cells and also on the transforming path from gametophyte to sporophyte for pollen cells.