Effects of atropine treatment on cortical,striatal and hippocampal high-affinity uptake of choline in the mouse

Changes in high-affinity uptake of choline (H.A._Ch) were studied in synaptosomes from different mouse brain regions following intravenous (i.v.) administration of atropine (0.3–30 mg/kg body weight) $\text{in vivo}$. The Ch-uptake was expressed as a Ch-uptake index, defined as the ratio between H.A._Ch and the corresponding choline acetylt-ransferase (ChAt) activity. The Ch-uptake index was highest in the hippocampus and lowest in the striatum. In the hippocampus a dose-dependent increase in this index was found following atropine treatment, while the striatal Ch-uptake index was unaffected by atropine. Atropine given i.v. in a dose of 10 mg/kg induced a 86% increase in V_max in synaptosomes from the hippocampus.