Inhibition of rabbit neutrophil lysosomal enzyme secretion,non-stimulated and chemotactic factor stimulated locomotion by nordihydroguaiaretic acid

Nordihydroguaiaretic acid irreversibly inhibits both Ca⁺⁺ dependent and independent lysosomal enzyme release from rabbit peritoneal neutrophils induced by the chemotactic factors, formyl-methionyl-leucyl-phenylalanine and C5a in the presence of cytochalasin B. The inhibition is both concentration and time dependent. In addition, the cytochalasin B dependent release induced by arachidonic acid and the Ca⁺⁺ ionophore A23187 is similarly inhibited. Similar concentrations of NDGA also inhibit neutrophil locomotion and chemotactic factor enhanced locomotion, as measured using modified Boyden chambers. As nordihydroguaiaretic acid has been shown to be an inhibitor of lipoxygenase activity, it is possible that this pathway of arachidonic acid metabolism is important in neutrophil locomotion and in cytochalasin B dependent lysosomal enzyme release induced by secretagogues.