ELISA检测肿瘤患者血清64DP

据报道64DP是一种与肿瘤相关的人血清DNA结合蛋白质,其现有的定量测定方法略嫌繁琐粗糙。本文采用亲和层析法纯化的兔抗64DP抗体,建立了简便灵敏的酶免疫测定法(ELISA)。部分样本经ELISA和火箭电泳两种方法平行测定,证明两者有良好的相关性。对70例恶性肿瘤及86例正常对照血清的测定结果,肿瘤组平均64DP水平显著高于对照组,其中尤以肝癌、胃癌及肺癌的增高更为显著。对非肿瘤其它疾病患者的检测发现感染和外伤也能造成血清64DP的迅速增加。本研究表明血清64DP的改变不仅仅局限于恶性肿瘤,它具有急性期反应蛋白质的特征。这使64DP作为肿瘤标志物应用于临床受到一定限制。

DETECTION OF SERUM 64DP IN TUMOR PATIENTS BY ELISA

64DP is a kind of human serum DNA binding protein. Elevated content of 64DP in serum of patients with malignant diseases has been reported. But the assaying method used before for serum 64DP is complicated and insensitive. We have established a rapid and sensitive method-ELISA for quantitative assay of 64DP.24 samples were parellelly examined by ELISA and rocket electrophoresis. The two methods showed good correlation. The serum 64DP in 70 patients with cancers and 83 controls were determined by ELISA. The result showed that the serum 64DP level in tumor group was significantly higher than control group. Among various cancers, the liver cancer, stomach cancer and lung dancer had a higher serum 64DP level. Patients with non-neoplastic diseases, such as inflammation and trauma were examined. Their serum 64DP level were also elevated. The result showed that 64DP was not a tumor specific serum protein, but rather an acute phase reactant protein. This finding provided anothe.r evidence for the similarity between 64DP and α1-antichymotrypsin.