Induction of the soxRS regulon of Escherichia coli by glycolaldehyde |
| |
Authors: | Benov Ludmil Fridovich Irwin |
| |
Institution: | Department of Biochemistry, Faculty of Medicine, Kuwait University, P.O. Box 24923, Safat, Kuwait. lbenov@hsc.kuniv.edu.kw |
| |
Abstract: | The ability of short-chain sugars to cause oxidative stress has been examined using glycolaldehyde as the simplest sugar. Short-chain sugars autoxidize in air, producing superoxide and alpha,beta-dicarbonyls. In Escherichia coli the soxRS regulon mediates an oxidative stress response, which protects the cell against both superoxide-generating agents and nitric oxide. In superoxide dismutase-deficient E. coli mutants, glycolaldehyde induces fumarase C and nitroreductase A, which are regulated as members of the soxRS regulon. A mutational defect in soxRS eliminates that induction. This establishes that glycolaldehyde can cause induction of this defensive regulon. This effect of glycolaldehyde was oxygen-dependent, was not shown by glyoxal, and was not seen in the superoxide dismutase-replete parental strain, and it was abolished by a cell-permeable SOD mimetic. All of these suggest that superoxide radicals produced by the oxidation of glycolaldehyde played a key role in the induction. |
| |
Keywords: | Induction of the soxRS regulon Glycolaldehyde autoxidation Short-chain sugars Oxidative stress Fumarase C Nitroreductase A |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|