(1) Experimental Medicine and Biotechnology Department, Postgraduate Institute of Medical Education and Research, 160 012 Chandigarh, India
Abstract:
The study, addressed to understand whether or not human platelets possess a unique thiol-oxidase whose activity could be modulated by signalling pathway initiated upon the activation of Receptor-Ck revealed the existence of disulphide-dependent oxidation within these cells and this phenomenon was regulated by Receptor-Ck-dependent generation of second messengers especially phosphatidic acid (PA); cAMP and cGMP. Purification of this activity revealed the existence of 47 kDa protein having thiol-oxidase activity. Keeping in view these results we propose that the existence of this novel 47 kDa Thiol-oxidase within human platelets may provide a crucial switch for the regulation of Receptor-Ck-dependent mevalonate pathway in human platelets.