Long-term survival of the plant-growth-promoting bacteria Azospirillum brasilense and Pseudomonas fluorescens in dry alginate inoculant |
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Authors: | Y Bashan L E Gonzalez |
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Institution: | (1) Department of Microbiology, The Center for Biological Research of the Northwest (CIB), La Paz, P.O. Box 128, B.C.S. 23000, Mexico e-mail: bashan@cibnor.mx Fax: +52-112-54710 or -53625, MX;(2) Department of Biology, Pontificia Universidad Javeriana, Apartado Aereo 56710, Santafe de Bogota, Colombia, CO |
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Abstract: | Two plant-growth-promoting bacteria, Azospirillum brasilense Cd and Pseudomonas fluorescens 313, immobilized in 1983 in two types of alginate-bead inoculant (with and without skim-milk supplement) and later dried
and stored at ambient temperature for 14 years, were recovered in 1996. The population in each type of bead had decreased,
yet significant numbers survived (105–106 cfu/g beads). Population numbers depended on the bead type and the three independent bacterial counting methods: the conventional
plate-count method, indirect enzyme-linked immunosorbent assay and the limited-enrichment technique. Both bacterial species
retained several of their original physiological features. When inoculated onto wheat plants, both species colonized and produced
plant-growth effects equal to those of the contemporary strain from a culture collection or to their own 1983 records. This
study showed that bacteria can survive in alginate inoculant over long periods.
Received: 1 May 1998 / Received revision: 24 August 1998 / Accepted: 3 September 1998 |
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