Characterization of gene expression of <Emphasis Type="Italic">QM</Emphasis> from <Emphasis Type="Italic">Caragana jubata</Emphasis>, a plant species that grows under extreme cold |
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Authors: | Pardeep Kumar Bhardwaj Paramvir Singh Ahuja Sanjay Kumar |
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Institution: | (1) Biotechnology Division, Institute of Himalayan Bioresource Technology, Council of Scientific and Industrial Research, P.O. Box 6, Palampur, Himachal Pradesh, 176061, India; |
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Abstract: | Caragana Caragana jubata (Pall.) Poir] is a temperate plant that thrives well under extremes of cold in high altitude of Himalaya and hence the plant
is expected to be a source of genes that might play an important role in tolerance to low temperature (LT). In order to identify
LT inducible gene(s), differential display of mRNA (DD) was performed using the apical buds growing under snow as well as
growing in the near vicinity without snow, and a LT inducible QM gene (CjQM) homologue was identified. Realizing the importance of QM gene (which encodes human Wilms’ tumor suppressor QM protein) in aggregation of 40 and 60S ribosomal subunit and that not
much has been reported on this gene in plant systems in relation to its relationship with LT, full length cDNA of CjQM was cloned through rapid amplification of cDNA ends. The gene (977 bp), encoded by small gene family, had an open reading
frame of 651 bp and was found to be intronless. The gene exhibited up-regulation within 20 min of exposure to LT and abscisic
acid (ABA), but no significant change in gene expression was observed in response to drought stress (DS), salicylic acid (SA)
and methyl jasmonate (MJ) application. Up-regulation of CjQM was obtained in the tissues growing in situ under snow. Non-responsiveness of CjQM towards DS, SA and MJ, but up-regulation in response to LT and ABA suggested a specific regulation of the gene in Caragana
under varied cues. |
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