Interleukin 6 modulation of second messenger systems in anterior pituitary cells. |
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| Authors: | M Grimaldi O Meucci A Scorziello T Florio C Ventra R De Mercato G Schettini |
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| Institution: | Dip. di Scienza delle Comunicazioni Umane, II Facoltà di Medicina e Chirurgia, Università di Napoli, Italy. |
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| Abstract: | We investigated the effect of interleukin-6 (IL-6) on second messenger systems in anterior pituitary (AP) cells. The acute exposition of membranes derived from the pituitary gland to IL-6 did not modify basal and forskolin-stimulated adenylate cyclase (AC) activity, as well as inositol phosphate (IP) production and free Ca(++)]i. Preincubation of AP cells with IL-6 for 20 min did not affect basal second messengers levels, while completely abolished the stimulation by VIP of AC activity, partially inhibited forskolin-stimulated cAMP formation and reduced TRH-stimulated IP production. Finally, the pretreatment of AP cells for 20 min with IL-6 also reduced the TRH-induced rise in free Ca(++)]i. |
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