转mapk双链RNA干扰表达载体黄瓜对根际土壤细菌多样性的影响

随着RNA干扰技术的发展,通过植物表达病原物特异的dsRNA来防治植物病害的转基因作物越来越多.转根结线虫mapk双链RNA表达载体的黄瓜能够通过RNA干扰作用沉默线虫的mapk基因,对根结线虫具有良好的防治效果.为了评价该转基因黄瓜的安全性,明确mapk双链RNA干扰表达载体转基因黄瓜植株对根际土壤细菌多样性的影响,采用16S rRNA基因克隆文库方法对非转基因黄瓜和转基因黄瓜土壤细菌群落多样性进行分析.结果表明,非转基因黄瓜土壤细菌文库包含124个OTU(可操作分类单元),转基因黄瓜土壤细菌文库包含122个OTU.2个文库共同拥有的OTU为115个.2个文库都包含1 3个类群细菌,Acidobacteria、Actinobacteria、Armatimonadetes、Bacteroidetes、candidate division BRC1、Chloroflexi、Firmicutes、Gemmatimonadetes、Nitrospira、Planctomycetes、Proteobacteria、Verrucomicrobia、unclassified_Bacteria. 其中 Proteobacteria、Bacteroidetes 、Chloroflexi和Acidobacteria是优势菌群.其他细菌类群数量相对较少.在纲分类水平上,两个文库包含的细菌类群一致,且各类群细菌比例差异不大.在Acidobacteria门中,Acidobacteria_Gp6为优势菌群.在Bacteroidetes门中,Sphingobacteria纲细菌数量最多.在Chloroflexi门中,unclassified Chloroflexi细菌最多.在Proteobacteria门中,其中Betaproteobacteria纲的细菌数量最多.从多样性指数角度分析,两种土壤细菌群落的Shannon、Simpson和Chao值差异不大.总体来看,两种土壤细菌类群差异不显著,转基因黄瓜未对根际土壤细菌群落产生明显影响.

The effect of transgenic cucumber with double strands RNA of mapk on diversity of rhizosphere bacteria

With the development of RNA interference, transgenic crops with double strands RNA to control plant disease are more and more. The transgenic cucumber with mapk (Mitogen-activated protein kinase) dsRNA could inhibit expression of mapk gene of root knot nematode, and had good control efficiency to the nematode. In order to evaluate the ecological safety of this transgenic cucumber and to reveal the effect on the community of soil bacteria, the diversity of soil bacteria in non-transgenic cucumber and transgenic cucumber were revealed by 16S rRNA gene clone library. The result showed that 124 OTUs were obtained from the library of non-transgenic cucumber soil, and 122 OTUs were found from the library of transgenic cucumber soil. 115 OTUs were shared by two libraries. The bacteria in two libraries were belonged to Acidobacteria, Actinobacteria, Armatimonadetes, Bacteroidetes, candidate division BRC1, Chloroflexi, Firmicutes, Gemmatimonadetes, Nitrospira, Planctomycetes, Proteobacteria, Verrucomicrobia and unclassified_Bacteria. The bacteria of Proteobacteria, Bacteroidetes, Chloroflexi and Acidobacteria were dominant groups, and others groups were minority. At class level, the dominant bacteria groups obtained from two libraries were no difference, and the percentage of clones in each class was similar. In the phyla of Acidobacteria and Bacteroidetes, the dominant groups were Acidobacteria_Gp6 and Sphingobacteria. The bacteria of unclassified Chloroflexi and Betaproteobacteria were majority in the phyla of Chloroflexi and Proteobacteria. From the index of diversity, there was no difference in two types of soil. On the whole, the bacterial communities of two types of soil were no difference, and not markedly affected by transgenic cucumber.