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The use of embryo culture for the recovery of plants from cassava (Manihot esculenta Crantz) seeds
Authors:Brenda J Biggs  Michael K Smith  Kenneth J Scott
Institution:(1) Department of Biochemistry, University of Queensland, 4067 St Lucia, Qld, Australia
Abstract:Cassava fertility and seed viability are frequently low, which can be a disadvantage in a breeding programme. An embryo culture method is described whereby embryonic axes are excised from mature seeds and placed on a culture medium containing 1.23 mgrM indolebutyric acid (IBA) at 30°C under continuous light. The number of plants recovered by embryo culture was much greater than the number recovered from conventional seed germination procedures.
Keywords:Manihot esculenta  cassava  embryo culture  seed germination
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