Transmural distribution of iron in the hypoxic and reoxygenated rabbit left ventricular myocardium |
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Authors: | Domenico Lapenna Giuliano Ciofani Sante D Pierdomenico Mario Di Gioacchino Matteo Neri Maria Adele Giamberardino Enrico Sabbioni Franco Cuccurullo |
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Institution: | Department of Medicine and Science of Aging, 'G. d'Annunzio' University School of Medicine and 'G. d'Annunzio' University Foundation, 66100, Chieti, Italy. |
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Abstract: | Transmural distribution of low molecular weight iron (LMWI), total iron, and protein carbonyls (PC) was investigated in the perfused rabbit heart under aerobic conditions, and after 60 min hypoxia followed or not by 3 min reoxygenation. In the aerobic perfused hearts, LMWI, total iron and PC did not show significant transmural differences. Hypoxia increased LMWI and PC levels, which were significantly higher in the subendocardium than in the subepicardium; further significant changes were not observed after reoxygenation. Total iron showed no transmural difference and was not significantly affected by both hypoxia and reoxygenation. Free iron was undetectable in the myocardial effluent of all experimental groups. Thus, hypoxia favors myocardial iron decompartmentalisation and oxidative stress, which are significantly greater in the inner than in the outer ventricular layers. Such findings may add further insight into the problem of the vulnerability of the mammalian subendocardium to injury induced by oxygen deprivation. |
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Keywords: | subendocardium subepicardium hypoxia iron free radicals oxidative stress |
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