Ratio Spectra Derivative and Zero-Crossing Difference Spectrophotometric Determination of Olmesartan Medoxomil and Hydrochlorothiazide in Combined Pharmaceutical Dosage Form |
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Authors: | Ambadas R Rote Pankaj D Bari |
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Institution: | (1) Department of Pharmaceutical Chemistry, M.G.V’s Pharmacy College, Mumbai-Agra Highway, Panchavati, Nashik, 422003, India |
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Abstract: | Two simple, economical, rapid, precise, and accurate methods for simultaneous determination of olmesartan medoxomil and hydrochlorothiazide
in combined tablet dosage form have been developed. The first method is based on ratio spectra derivative spectrophotometry,
and the second method is zero-crossing difference spectrophotometry. The amplitudes in the first derivative of the corresponding
ratio spectra at 231.0 and 271.0 nm were selected to determine olmesartan medoxomil and hydrochlorothiazide, respectively.
Measurements of absorbance were carried out at zero-crossing wavelengths 257.8 and 240.2 nm for olmesartan medoxomil and hydrochlorothiazide
by zero-crossing difference spectrophotometric method. Beer’s law is obeyed in the concentration range of 08–24 μg/mL for
olmesartan medoxomil (OLM) and 05–15 μg/mL for hydrochlorothiazide (HCT) by ratio spectra derivative and 05–30 μg/mL for OLM
and HCT by zero-crossing difference spectrophotometric method. The results of the assay were found to be 100.46 ± 0.95 for
OLM and 100.4 ± 0.27 for HCT by ratio spectra derivative and 99.06 ± 1.14 for OLM and 100.05 ± 0.90 for HCT by zero-crossing
difference spectrophotometric method. These methods passes F test and t test. Both methods were validated statistically and by performing recovery study. |
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