| 高产苯基乙酰基甲醇菌株选育 |
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| 引用本文： | 李继珩,尚广东. 高产苯基乙酰基甲醇菌株选育[J]. 氨基酸和生物资源, 2001, 23(1): 16-20 |
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| 作者姓名： | 李继珩 尚广东 |
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| 作者单位： | 中国药科大学生物制药教研室，南京 210009 |
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| 基金项目： | 国家科技攻关项目;JBO2-902-07; |
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| 摘 要： | 研究发现 1 0种酵母菌株均可将苯甲醛转化为PAC ,且苯甲醛对所有酵母的最小抑制作用均在 0 .2 %～0 .4 %之间。采用温度、uV、DES、LiCI以及LiCI和uV联合诱变结果 ,获得 3个转化活力较高的变异株 ,其中变异株S .CarlebergensisCPU 950 7 4 3″连续接种培养五代并进行转化和测试 ,结果转化活力均较高 ,表明变异株遗传稳定性良好。
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| 关 键 词： | 酵母 诱变 苯甲醛 PAC |
| 文章编号： | 1006-8376(2001)-0016-05 |
| 修稿时间： | 2000-06-01 |
Selection of Phenysacetycarbinol-producing yeast Strain |
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| LI Ji-hen,SHANG Guang-dong.. Selection of Phenysacetycarbinol-producing yeast Strain[J]. Amino Acids & Biotic Resources, 2001, 23(1): 16-20 |
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| Authors: | LI Ji-hen SHANG Guang-dong. |
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| Abstract: | It was found that all of ten yeast strains studied in this papercould transform benzaldhyde into phenylacetycarbinol (PAC). The inhibitive concentration of benzaldhyde on all these yeast strains was between 0.2%-0.4%. With UV,DES,LiCI and UV combining with LiCI as mutagens, three mutants of the yeast with high transformation efficiency were obtained, of which the S. Carlebergensis CPU 9507-4-3” was inoculated and cultivated sequentially for 5 generation for transformation tests. The results indicated that the strains was genetically stable and could efficiently transform benzaldhyde into PAC with high transformation efficiency. |
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| Keywords: | Yeast Mutation Benzaldhy PAC |
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