The role of stem cells in midgut growth and regeneration |
| |
Authors: | Hakim R S Baldwin K M Loeb M |
| |
Institution: | (1) Department of Anatomy, College of Medicine, Howard University, 520 W. Street, 20059 NW Washington, DC;(2) Insect Biocontrol Laboratory, Building 011A, Room 211 BARC WEST, 20705 Beltsville, Maryland |
| |
Abstract: | Summary TheManduca sexta (L.) Lepidoptera: Sphingidae] andHeliothis virescens (F.) Lepidoptera: Noctuidae] midguts consist of a pseudostratified epithelium surrounded by striated muscle and tracheae.
This epithelium contains goblet, columnar, and basal stem cells. The stem cells are critically important in that they are
capable of massive proliferation and differentiation. This growth results in a fourfold enlargement of the midgut at each
larval molt. The stem cells are also responsible for limited cell replacement during repair. While the characteristics of
the stem cell population vary over the course of an instar, stem cells collected early in an instar and those collected late
can start in vitro cultures. Cultures of larval stem, goblet, and columnar cells survive in vitro for several mo through proliferation
and differentiation of the stem cells. One of the two polypeptide differentiation factors which have been identified and characterized
from the culture medium has now been shown to be present in midgut in vivo. Thus the ability to examine lepidopteran midgut
stem cell growth in vitro and in vivo is proving to be effective in determining the basic features of stem cell action and
regulation.
Mention of any product in this publication does not imply endorsement by the USDA. |
| |
Keywords: | stem cell midgut Lepidoptera differentiation factors |
本文献已被 PubMed SpringerLink 等数据库收录! |
|