一个来自硬粒小麦的抗白粉病基因的鉴定和微卫星标记

在起源于硬粒小麦(TriticumdurumDesf.accessionDR147)和尾状山羊草(AegilopscaudataL.acc.Ae14)合成的双二倍体与普通小麦品种“莱州953”杂交组合衍生的BC3F2群体中鉴定了一个抗小麦白粉病基因。遗传分析表明,该基因为一个显性单基因。应用分离群体分组法(BSA),鉴定了两个与抗病基因紧密连锁的微卫星标记Xgwm311和Xgwm382,它们与抗病基因的遗传距离分别为5.9cM和4.9cM。对双二倍体亲本硬粒小麦DR147和尾状山羊草Ae14及轮回亲本“莱州953”的DNAPCR扩增结果表明,与抗病基因相关的微卫星标记Xgwm311和Xgwm382来源于硬粒小麦DR147。根据已发表的小麦微卫星图谱和对“中国春”缺-四体系DNA扩增结果,抗病基因被定位在小麦2A染色体的长臂末端。

Identification and Microsatellite Markers of a Resistance Gene to Powdery Mildew in Common Wheat Introgressed from Triticum durum

A powdery mildew resistance gene in a BC3F2 population, derived from a cross made betweenan amphidiploid of Triticum durum Desf.-Aegilops caudata L. and T. aestivum L. cv. Laizhou 953, wasidentified. Genetic analysis of resistance to powdery mildew in BC3F2 population and derived BC3F3 familiesindicated a single dominant gene controlled the resistance. By bulk segregation analysis, two microsatellitemarkers, Xgwm311 and Xgwm382, were identified to be closely linked to the resistance gene with geneticdistance of 5.9 cM and 4.9 cM, respectively. DNA from T. durum accession DR147, Ae. caudata acc. Ae14,and recurrent parent wheat (Triticum aestivum L.) cv. Laizhou 953 were amplified with primer pairs WMS311and WMS382, the specific bands related to the resistance gene were only present in T. durum acc. DR147.Results showed that the resistance gene originated in T. durum acc. DR147. Based on the location of thelinked microsatellite markers, the resistance gene was located on the telomeric region of chromosome2AL in wheat. Temporarily, the resistance gene was designated as PmDR147. The relation of this geneand Pm4 was discussed.