Effects of NO
3
deficiency and NaCl stress on suberin deposition in rhizo- and hypodermal (RHCW) and endodermal cell walls (ECW) of castor bean (Ricinus communis L.) roots |
| |
Authors: | Lukas Schreiber Rochus Franke Klaus Hartmann |
| |
Institution: | (1) Department of Ecophysiology, Institute of Cellular and Molecular Botany, University of Bonn, Kirschallee 1, D-53115 Bonn, Germany |
| |
Abstract: | Castor bean (Ricinus communis L.) plants were hydroponically cultivated to achieve NO3 deficiency (N starvation), salt stress (addition of 100 mM NaCl), or normal conditions. Endodermal (ECW) and rhizodermal
and hypodermal cell walls (RHCW) were isolated enzymatically from roots, and suberin monomers were released by transesterification
after solvent extraction. Aromatic and aliphatic suberin monomers were identified and quantified by gas chromatography and
mass spectrometry. Between 90 and 95% of the released suberin monomers were linear, long-chain, aliphatic compounds (alcohols,
acids, diacids, ω-hydroxy acids and 2-hydroxy acids) with an average chain length of 19 C-atoms. The remainder was an aromatic
suberin fraction mainly composed of coumaric and ferulic acid. Suberin amounts were significantly increased in ECW and RHCW
in the presence of NaCl. In contrast, N starvation led to significantly reduced levels of suberization in ECW and RHCW. It
is concluded that R. communis plants reinforce their apoplastic transport barriers in roots in adaptation to NaCl stress in order to minimize NaCl uptake.
Under conditions of N starvation the opposite occurs and plants reduce the suberization of their apoplastic transport barriers
to facilitate nutrient uptake form the soil. |
| |
Keywords: | apoplast Casparian strip endodermis hypodermis R communis root suberin |
本文献已被 SpringerLink 等数据库收录! |
|