Targeted modulation of sinapine biosynthesis pathway for seed quality improvement in Brassica napus |
| |
Authors: | V-S Bhinu Ulrike A Schäfer Rong Li Jun Huang Abdelali Hannoufa |
| |
Institution: | (1) Genomics and Biotechnology, Agriculture and Agri-Food Canada, Saskatoon Research Centre, 107 Science Place, Saskatoon, SK, Canada, S7N 0X2 |
| |
Abstract: | Arabidopsis thaliana and other members of the Brassicaceae accumulate the hydroxycinnamic acid esters sinapoylmalate in leaves and sinapoylcholine
in seeds. Our recent understanding of the phenylpropanoid pathway although complex has enabled us to perturb the sinapine
biosynthesis pathway in plants. Sinapine (sinapoylcholine) is the most abundant antinutritional phenolic compound in seeds
of cruciferous species and therefore is a target for elimination in canola (Brassica napus) meal. We analysed A. thaliana mutants with specific blocks in the phenylpropanoid pathway and identified mutant lines with significantly altered sinapine
content. Knowledge gained from A. thaliana was extended to B. napus and the corresponding phenylpropanoid pathway genes were manipulated to disrupt sinapine biosynthesis in B. napus. Based on our understanding of the A. thaliana genetics, we have successfully developed transgenic B. napus lines with ferulic acid 5-hydroxylase (FAH) and sinapoylglucose:choline sinapoyltransferase (SCT)-antisense. These lines with concomitant downregulation of FAH and SCT showed up to 90% reduction in sinapine. In addition
to reduced sinapine content, we detected higher levels of free choline accumulation in the seeds. These results indicate that
it is possible to develop plants with low sinapine and higher choline by manipulating specific steps in the biosynthetic pathway.
These improvements are important to add value to canola meal for livestock feed.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. |
| |
Keywords: | Brassica Canola Choline Phenylpropanoid RNAi Sinapine |
本文献已被 PubMed SpringerLink 等数据库收录! |
|