Methods for the analysis of protein-chromatin interactions |
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Authors: | Sarah J Brickwood Fiona A Myers Dr Simon P Chandler |
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Institution: | (1) Sangamo Biosciences Inc., Point Richmond Tech Center II, 501 Canal Blvd., Suite A100, 04804 Richmond, CA |
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Abstract: | The analysis of protein interactions with chromatin is vital for the understanding of DNA sequence recognition in vivo. Chromatin
binding requires the interaction of proteins with DNA lying on the macromolecular protein surface of nucleosomes, a situation
that can alter factor binding characteristics substantially when compared with naked DNA. It is therefore important to study
these protein-DNA interactions in the context of a chromatin substrate, the more physiologically relevant binding situation.
In this article we review techniques used in the investigation of protein interactions with defined nucleosomal templates. |
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