Large-scale synthesis of the mRNA analogue C17AUGA4C17 |
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| Authors: | A Gnirke K H Nierhaus |
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| Affiliation: | Max-Planck-Institut für Molekulare Genetik, Abteilung Wittmann, Berlin-Dahlem. |
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| Abstract: | The large-scale synthesis of the mRNA analogue C17AUGA4C17 is described. This molecule is 41 bases in length and contains three unique codons in its central region. tRNA binding studies at 6 mM Mg2+ in the absence and presence of the labeled heteropolymer show the mutual stimulation of tRNA and mRNA binding and demonstrate that this mRNA provides a simple and suitable system for the analysis of the reactions of the ribosomal elongation cycle. |
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