A general synthesis of specifically deuterated nucleotides for studies of DNA and RNA |
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Authors: | Chen Bingzi Jamieson Elizabeth R Tullius Thomas D |
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Affiliation: | Department of Chemistry, Boston University, Boston, MA 02215, USA. |
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Abstract: | An efficient procedure is described for the preparation of ribonucleotides and deoxyribonucleotides with deuterium incorporated at the 1', 4', or 5' position. Three intermediates-[1-2H]-D-ribose, [4-2H]-D-ribose, and [5-2H(2)]-D-ribose-were prepared by chemical synthesis and subsequently converted to ribonucleotides and deoxyribonucleotides via enzymatic reactions. Milligram quantities of the desired products were obtained with an average deuterium content of 96+/-1%. |
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