The role of the DRY motif of human MC4R for receptor activation |
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| Authors: | Yamano Yoshiaki Kamon Rio Yoshimizu Takao Toda Yoshihisa Oshida Yuichi Chaki Shigeyuki Yoshioka Masanobu Morishima Isao |
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| Institution: | Department of Biochemistry and Biotechnology, Faculty of Agriculture, Tottori University, Tottori, Japan. yyamano@muses.tottori-u.ac.jp |
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| Abstract: | We constructed several mutant human MC4R cDNAs by site directed mutagenesis and expressed these receptors in COS-1 cells. The conserved DRY motif among GPCRs was mutated to generate eight mutants. While no MC4R ligand binding was detected in any of the mutants, one mutant, D146A, resulted in higher cAMP production in cells than the wild-type receptor without ligand stimulation. |
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