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Isolation and Characterization of a Novel Thraustochytrid-like Microorganism that Efficiently Produces Docosahexaenoic Acid |
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| Authors: | Zakia Perveen Hitomi Ando Akio Ueno Yukiya Ito Yusuke Yamamoto Yohko Yamada Tomoko Takagi Takako Kaneko Kazuhiro Kogame Hidetoshi Okuyama |
| Institution: | (1) Laboratory of Environmental Molecular Biology, Graduate School of Environmental Earth Science, Hokkaido University, Sapporo Kita-ku, 060-0810, Japan;(2) ROM Co. Ltd., Chuo-ku, Sapporo 064-0804, Japan;(3) Department of Chemical and Biological Sciences, Faculty of Science, Japan Women’s University, 112-8681 Bunkyo-ku, Japan;(4) Division of Biology, Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Meguro-ku, Tokyo 153-8902, Japan;(5) Division of Biological Sciences, Graduate School of Science, Hokkaido University, Kita-ku, Sapporo 060-0810, Japan |
| Abstract: | A thraustochytrid-like microorganism (strain 12B) was isolated from the mangrove area of Okinawa, Japan. On the basis of its ectoplasmic net structure and biflagellate zoospores we determined strain 12B to be a novel member of the phylum Labyrinthulomycota in the kingdom Protoctista. When grown on glucose/seawater at 28 °C, it had a lipid content of 58% with docosahexaenoic acid (DHA; 22:6 n−3) at 43% of the total fatty acids. It had a growth rate of 0.38 h−1. The DHA production rate of 2.8 ± 0.7 g l−1 day−1 is the highest value reported for any microorganism. Received 7 October 2005; Revisions requested 7 October 2005; Revisions received 15 November 2005; Accepted 15 November 2005 |
| Keywords: | docosahexaenoic acid (DHA) Labyrinthulomycota polyunsaturated fatty acid (PUFA) |
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