Characterization of the purified multifunctional cellulase component of Penicillium funiculosum |
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Authors: | N A Sahasrabudhe A H Lachke P K Ranjekar |
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Institution: | (1) Division of Biochemical Sciences, National Chemical Laboratory, 411 008 Pune, India |
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Abstract: | Summary The endoglucanase component (CMCase I) ofPenicillium
funiculosum cellulase was purified to apparent homogeneity by ultrafiltration and gel chromatography. It consists of a single polypeptide chain with a molecular weight of 56000 and is a glycoprotein. Viscometric and end-product analysis revealed the randomness of enzyme action. Multifunctional characteristic of CMCase I was studied with various carbohydrate substrates.NCL Communication No.: 4307 |
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