| ELISA测定中TMB显色体系的优化及其稳定性研究 |
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| 引用本文： | 李红梅,陈佳,徐斐,曹慧,Maria Eugenia. ELISA测定中TMB显色体系的优化及其稳定性研究[J]. 生物技术通报, 2010, 0(2) |
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| 作者姓名： | 李红梅 陈佳 徐斐 曹慧 Maria Eugenia |
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| 作者单位： | 上海理工大学医疗器械与食品学院,上海,200093 |
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| 基金项目： | 上海市教委重点攻关项目 |
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| 摘 要： | 通过对间接酶联免疫测定中显色体系的各影响因素的研究,确定了酶联免疫测定的最优显色体系为二甲基亚砜(DMSO)配制四甲基联苯胺(TMB)浓度为0.3 mg/mL的溶液为A液,pH5.5,0.2 mol/L磷酸氢二钠与0.1 mol/L柠檬酸缓冲液配制过氧化脲素浓度为0.74 mg/mL的溶液为B液,两者按一定比例混合后使用。试验结果表明,此显色体系在25-40℃内进行都可得到较为准确的结果,且A、B液混合后在室温下放置4 h仍可使用。A液及B液分别在4℃放置60 d后混合使用仍不影响测定结果。
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| 关 键 词： | 酶联免疫测定 显色 辣根过氧化物酶 |
Optimization of TMB Substrate Chromogenic System in ELISA and the Study of Its Stability in Storage |
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| Li Hongmei,Chen Jia,Xu Fei,Cao Hui,Maria Eugenia. Optimization of TMB Substrate Chromogenic System in ELISA and the Study of Its Stability in Storage[J]. Biotechnology Bulletin, 2010, 0(2) |
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| Authors: | Li Hongmei Chen Jia Xu Fei Cao Hui Maria Eugenia |
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| Affiliation: | Li Hongmei Chen Jia Xu Fei Cao Hui Maria Eugenia(School of Medical Instrument , Food Engineering,University of Shanghai for Science , Technology,Shanghai 200093) |
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| Abstract: | By the study of the factors which influence the chromogenic reaction in indirect ELISA,the optimal chromogenic system was obtained.Solution A was prepared by TMB dissolved in DMSO with the concentration of 0.3 mg/mL,solution B was prepared by hydrogen peroxide urea dissolved in pH5.5,0.2 mol/L Na2HPO4-0.1 mol/L citric acid buffer with the concentration of 0.74 mg/mL,and the substrate chromogenic system was prepared by mixing A and B in certain ratio.The experiment results showed that this substrate chromoge... |
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| Keywords: | TMB ELISA Chromogenic HRP TMB |
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