| 耐高浓度甘油的1,3-丙二醇产生菌诱变选育与固定化培养初步研究 |
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| 引用本文： | 彭益强,兰琳,方柏山,张文珍. 耐高浓度甘油的1,3-丙二醇产生菌诱变选育与固定化培养初步研究[J]. 激光生物学报, 2007, 16(6): 754-758 |
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| 作者姓名： | 彭益强 兰琳 方柏山 张文珍 |
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| 作者单位： | 华侨大学,工业生物技术福建省高等学校重点实验室,福建,泉州,362021;华侨大学,信息科学与工程学院,福建,泉州,362021 |
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| 基金项目： | 国家“973”计划项目(2007CB707804)，国家自然科学基金项目(20446004,20676048)，国务院侨办课题(06QZR08) |
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| 摘 要： | 紫外诱变筛选耐高浓度甘油的1,3-PD克雷伯杆菌,诱变条件为30 W紫外灯,距离34 cm,照射6 m in,平板涂布稀释度为10-5,经六轮诱变筛选出耐90 g/L甘油的变异菌株,甘油转化率稳定在45%左右,1,3-PD生成量稳定在40 g/L左右,比原始出发菌株提高了近30%,诱变菌株经考察具有一定的遗传稳定性。在此基础上应用溶胶-凝胶法进行固定化细胞实验并与游离细胞进行了四个批次的对比连续发酵,结果显示溶胶-凝胶法具有一定的稳定性。
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| 关 键 词： | 1 3-丙二醇 诱变 溶胶-凝胶 固定化 |
| 文章编号： | 1007-7146（2007）06-0754-05 |
| 收稿时间： | 2007-01-20 |
| 修稿时间： | 2007-01-20 |
The Selection of 1,3-propanediol Produced Bacterium Enduring High Glycerol Concentration Mutate and Its Immobidi Ferment Study |
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| PENG Yi-qiang,LANG Lin,FANG Bai-shan,ZHANG Wen-zhen. The Selection of 1,3-propanediol Produced Bacterium Enduring High Glycerol Concentration Mutate and Its Immobidi Ferment Study[J]. ACTA Laser Biology Sinica, 2007, 16(6): 754-758 |
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| Authors: | PENG Yi-qiang LANG Lin FANG Bai-shan ZHANG Wen-zhen |
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| Abstract: | The 1,3-propanediol(1,3-PD) produced Klebsiella pneumoniae that endure high concentration glycerol(Gly) was flitrated by ultraviolet mutate,the suit mutate condition was 30 W ultraviolet,space 34 cm,irradiate 6 min,dilute 10-5 to smear.After 6 cycle mutate,a bacterium that endure 90 g/L glycerol and had some have certain inheritance stability was filtrated,glycerol translation rate was 45 %,1,3-PD production was about 40 g/L,improved about 30 % compared to originality bacterium.The cell immobilized by sol-gel was fermentioned to produce 1,3-PD and showed certain stability after 4 fermention batchs. |
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| Keywords: | 1 3-propanediol mutant sol-gel immobilize |
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