Identification of secreted proteins of Aspergillus oryzae associated with growth on solid cereal substrates |
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Authors: | Te Biesebeke R Boussier A van Biezen N van den Hondel C A M J J Punt P J |
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Affiliation: | Wageningen Centre for Food Sciences (WCFS), P.O. Box 557, 6700 AN, Wageningen, The Netherlands. tebiesebeke@yahoo.com |
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Abstract: | Filamentous growth of Aspergillus oryzae on solid cereal substrates involves secretion of substrate converting enzymes and a solid substrate specific polarised hyphal growth phenotype. To identify proteins produced under these specific conditions, the extracts of A. oryzae grown on wheat-based media were analysed using N-terminal sequence analysis. In a submerged wheat-based growth medium of A. oryzae, besides alpha-amylase, also an arabinosidase and xylanase were abundantly produced. In the extracts of A. oryzae grown on wheat-based solid substrate besides alpha-amylase and chitinase, two new proteins of 16 and 27 kDa were identified. These hypothetical proteins showed only close homologies to filamentous fungal proteins. |
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