Effect of triton X-100 on alkaline lipase production by Pseudomonas pseudoalcaligenes F-111 |
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| Authors: | Shuen-Fuh Lin Chien-Ming Chiou Ying-Chieh Tsai |
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| Institution: | (1) Department of Bioengineering, Tatung Institute of Technology, Taipei, Taiwan, Republic of China;(2) Institute of Biochemistry, National Yang-Ming University, Taipei, Taiwan, Republic of China |
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| Abstract: | Summary An extracellular alkaline lipase was evaluated from Pseudomonas pseudoalcaligenes F-111, which was isolated from soil using a rhodamine B agar plate with Na2CO3. For enzyme production, olive oil, soymeal, triton X-100 and sodium ion were found to be essential. The addition of triton X-100 increased the alkaline lipase by 50- fold. The effect of additives as well as the development of suitable culture conditions for lipase production is discussed. |
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