G-rich, a Drosophila selenoprotein, is a Golgi-resident type III membrane protein |
| |
Authors: | Chen Chang Lan Shim Myoung Sup Chung Jiyeol Yoo Hyun-Seung Ha Ji Min Kim Jin Young Choi Jinmi Zang Shu Liang Hou Xiao Carlson Bradley A Hatfield Dolph L Lee Byeong Jae |
| |
Affiliation: | School of Biological Sciences and Institute of Molecular Biology and Genetics, Seoul National University, Seoul 151-742, Republic of Korea. |
| |
Abstract: | G-rich is a Drosophila melanogaster selenoprotein, which is a homologue of human and mouse SelK. Subcellular localization analysis using GFP-tagged G-rich showed that G-rich was localized in the Golgi apparatus. The fusion protein was co-localized with the Golgi marker proteins but not with an endoplasmic reticulum (ER) marker protein in Drosophila SL2 cells. Bioinformatic analysis of G-rich suggests that this protein is either type II or type III transmembrane protein. To determine the type of transmembrane protein experimentally, GFP-G-rich in which GFP was tagged at the N-terminus of G-rich, or G-rich-GFP in which GFP was tagged at the C-terminus of G-rich, were expressed in SL2 cells. The tagged proteins were then digested with trypsin, and analyzed by Western blot analysis. The results showed that the C-terminus of the G-rich protein was exposed to the cytoplasm indicating it is a type III microsomal membrane protein. G-rich is the first selenoprotein identified in the Golgi apparatus. |
| |
Keywords: | Drosophila G-rich Golgi Microsomal membrane protein Selenium Selenoprotein SelK Type III |
本文献已被 ScienceDirect PubMed 等数据库收录! |