Searching for tagged male-sterile mutants of arabidopsis |
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| Authors: | Julie A Glover Katherina C Blömer Leigh B Farrell Abdul M Chaudhury Elizabeth S Dennis |
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| Institution: | (1) CSIRO Division of Plant Industry, GPO Box 1600, 2601 Canberra, ACT;(2) Cooperative Research Centre for Plant Science, PO Box 475, 2601 Canberra, ACT;(3) Division of Biochemistry and Molecular Biology, Australian National University, 0200 Canberra, ACT, Australia;(4) Present address: Research Unit, Peter MacCallum Cancer Institute, St Andrews Pl, 3002 East Melbourne, VIC, Australia;(5) Present address: Gene Shears Pty. Ltd, PO Box 1238, 1238 Neutral Bay, NSW, 2089, Australia;(6) CSIRO Division of Plant Industry, GPO Box 1600, 2601 Canberra, ACT;(7) Cooperative Research Centre for Plant Science, PO Box 475, 2601 Canberra, ACT, Australia |
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| Abstract: | Although many male-sterile mutants have been identified inArbidopsis thaliana, few of the corresponding genes have been cloned. In order to facilitate cloning of a male sterility gene, 23 of Feldmann's
T-DNA-generated, reduced-fertility lines were screened to identify a tagged male-sterile mutation. Malesterile mutants were
identified, as well as mutants that were both male and female sterile. Segregation of the kanamycin marker gene in the progeny
of 15 of these lines was studied. Forty percent had functional T-DNAs (encoding resistance to kanamycin) inserted at a single
locus, the remainder segregating for two or more functional T-DNA inserts. Linkage between T-DNA inserts and mutant phenotype
was tested for six lines. In three of these lines, mutations were not linked to a T-DNA insert. In three lines, the mutation
segregated with a T-DNA insert. |
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| Keywords: | arabidopsis male-sterile mutant T-DNA |
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