Plaque size phenotype as a selectable marker to generate vaccinia virus recombinants. |
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| Authors: | J F Rodriguez and M Esteban |
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| Affiliation: | Department of Biochemistry, Health Science Center, State University of New York, Brooklyn 11203. |
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| Abstract: | In this report, we provide a new method for selection of vaccinia virus recombinants expressing foreign genes. The method is based on the use of the gene encoding the viral 14,000-molecular-weight envelope protein that rescues the small-plaque-size phenotype of a vaccinia virus variant to large-plaque-size virus. Selection of recombinants is easily obtained after visual inspection of large viral plaques. |
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