Simultaneous saccharification and fermentation of pretreated biomass: Improving mass balance closure

Summary The successful implementation of simultaneous saccharification and fermentation (SSF) technology for biomass conversion to ethanol requires competent analysis of complex biomass process streams, often obtained at extremes of pH. In this study, optimal conditions for handling biomass samples recovered from acid and alkaline pretreatments prior to traditional compositional analysis were developed. Methods for processing slurries from SSF were also determined. In both cases, a mixed wastepaper feedstock was used to test improved handling procedures and to document recommended performance.