Diamide blocks H+ conductance in mitochondrial H+-ATPase by oxidizing FB dithiol |
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Authors: | Y Huang M J Pringle D R Sanadi |
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Institution: | 1. Department of Cell Physiology, Boston Biomedical Research Institute, 20 Staniford Street, Boston, MA 02114 USA;2. Department of Biological Chemistry, Harvard Medical School, Boston, MA 02115, USA |
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Abstract: | Effects of diamide on proton conductance of electron transport particles (ETPH), purified H+-ATPase (F1-F0), F0 of the H+-ATPase from beef heart mitochondria and binding of cadmium (109Cd) to the H+-ATPase have been examined in the present paper. When ETPH and purified H+-ATPase are treated with 1 mM diamide, ATP-dependent generation of membrane potential, monitored by the absorbance change produced by the redistribution of oxonol VI, is consistently inhibited. Diamide also blocks passive H+ conductance driven by a K+ diffusion potential in the membrane sector, F0, of H+-ATPase. Furthermore, diamide treatment drastically reduces the binding of 109Cd2+ to H+-ATPase, showing competition for the FB dithiol group. |
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