High density culture of mouse-human hybridoma cells using a perfusion culture apparatus with multi-settling zones to separate cells from the culture medium |
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Authors: | Michiyuki Tokashiki Takami Arai |
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Institution: | (1) Biotechnology Research Laboratories, Tokyo Research Center, Teijin Limited, 4-3-2, Asahigaoka, 191 Hino-shi, Tokyo, Japan |
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Abstract: | Mouse-human hybridoma X87X cells were cultivated using a novel perfusion culture apparatus provided with three-settling zones to separate the cells from the culture medium by gravitational settling. The maximum viable cell density in a serum-free culture medium attained 3.0×107 cells/ml, when the specific perfusion rate was set to 2.3 vol day-1, and monoclonal antibody was continuously produced. These results were almost the same as those in the perfusion culture vessel with one settling zone and revealed that the process with a plurality of settling zones is a promising one for scale-up of a gravitation type of perfusion culture vessel. |
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Keywords: | hybridoma monoclonal antibody perfusion culture mammalian cell culture serum-free culture |
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