Isolation of putrescine-requiring mutants of Neurospora crassa |
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Authors: | K J McDougall Janet Deters Julie Miskimen |
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Institution: | (1) Department of Biology, University of Dayton, 45469 Dayton, Ohio, USA |
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Abstract: | Two auxotrophs of Neurospora crassa have been isolated that give a positive growth response to putrescine, spermidine or spermine. One of the mutants is deficient in ornithine decarboxylase activity and has been designated put-1. Both mutants map on linkage group VR, fail to complement and are infertile when crossed to one another, indicating that they are probably alleles. A putrescine auxotroph is incapable of suppressing a pro-4 mutant. The isolation of the mutants confirms that putrescine is an essential factor for the normal growth of the organism, and is synthesized via a single pathway in Neurospora. |
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